環保專案成果報告查詢系統

專案計畫編號	EPA-100-E3S4-02-01
經費年度	100
計畫經費	2,150 千元
專案開始日期	2011/01/31
專案結束日期	2011/12/31
專案主持人	陳家揚
主辦單位	環檢所
承辦人	陳麗霞
執行單位	臺灣大學
專案分類	環境監測及檢測
中文關鍵字	農藥;液相層析串聯式質譜儀;飲用水;表面水;固相萃取
英文關鍵字	Pesticides;LC-MS/MS;drinking water;Surface water;Solid-phase extraction
協同主持人
共同主持人	龍世俊
計畫聯絡人	陳家揚
計畫聯絡電話	02-33668101#
計畫聯絡信箱	dbms@ntu.edu.tw

中文摘要	農藥的施用範圍包含了農作物生產、雜草的控制以及消除對公共健康有害之生物。農藥施用後可經由雨水沖刷或空氣沈降至河川、地下水及土壤等環境中，故其對於水中生物或人體健康的影響值得關注。根據行政院農業委員會農藥原體之使用量資料，殺蟲劑、殺菌劑、除草劑三者為國內農藥使用之主要種類。本年度針對殺蟲劑選擇27種有機磷類、16種氨基甲酸鹽類、與7種合成除蟲菊精，共50種待測物，包括歐殺松(acephate)、谷速松(azinphos-methyl)、陶斯松(chlorpyrifos)、內吸磷(demeton)、滅賜松(demeton-S-methyl)、大利松(diazinon)、二氯松(dichlorvos)、大滅松(dimethoate)、二硫松(disulfoton)、普扶松(ethoprophos)、芬殺松(fenthion)、大福松(fonofos)、加福松(isoxathion)、馬拉松(malathion)、達馬拉(methamidophos)、滅大松(methidathion)、美文松(mevinphos)、亞素靈(monocrotophos)、巴拉松(parathion)、賽達松(phenthoate)、福瑞松(phorate)、易滅松(phosmet) 、佈飛松(profenofos)、喹硫磷(quinalphos)、亞培松(temephos)、托福松(terbufos)、三氯松(trichlorfon)、得滅克(aldicarb)、得滅克亞碸(aldicarb sulfoxide)、得滅克碸(aldicarb sulfone)、免扶克(benfuracarb)、加保利(carbaryl)、加保扶(carbofuran)、3-羥基加保扶(3-hydroxycarbofuran)、丁基加保扶(carbosulfan)、愛芬克(ethiofencarb)、丁基滅必蝨(fenobucarb)、滅必蝨(isoprocarb)、滅賜克(methiocarb)、納乃得(methomyl)、歐殺滅(oxamyl)、比加普(pirimicarb)、安丹(propoxur)、賽扶寧(cyfluthrin)、亞滅寧(cypermethrin)、第滅寧(deltamethrin)、芬普寧(fenpropathrin)、芬化利(fenvalerate)、百滅寧(permethrin)、福化利(tau-fluvalinate)，完成前處理技術開發以及液相層析/質譜/質譜儀分析方法建立。 500 mL水樣以Atlantic HLB(47 mm)固相圓盤萃取，以甲醇及二氯甲烷沖提，沖提液經濃縮至5 mL後，以極致液相層析儀(ultra-performance liquid chromatography, UPLC)搭配串聯式質譜儀以電灑游離（ESI）分析。液相層析管柱為Phenomenex Kinetex PFP(50 × 2.1 mm, 2.6 μm)。採樣地點為雲嘉南主要河川北港溪、八掌溪、及鹽水溪之重要支流以及其本流，以及一座水庫、一座淨水廠與一座生活污水處理廠，加上計劃委託單位另外交付之7個水體樣本，共計31個樣本。水體檢測結果顯示，北港溪流域檢出率較高，尤其是機磷類農藥，最高濃度為acephate(9.5 μg/L)。污水廠、淨水廠、水庫與計劃委託單位水樣之待測物濃度皆低於定量極限(LOQ, 500 ng/L)，且大多為未檢出。檢測結果與農藥原體之使用量有正向相關，使用量越高之農藥，待測物於水體中檢出率相對較高。
中文關鍵字	農藥;液相層析串聯式質譜儀;飲用水;表面水;固相萃取

中文摘要

農藥的施用範圍包含了農作物生產、雜草的控制以及消除對公共健康有害之生物。農藥施用後可經由雨水沖刷或空氣沈降至河川、地下水及土壤等環境中，故其對於水中生物或人體健康的影響值得關注。根據行政院農業委員會農藥原體之使用量資料，殺蟲劑、殺菌劑、除草劑三者為國內農藥使用之主要種類。本年度針對殺蟲劑選擇27種有機磷類、16種氨基甲酸鹽類、與7種合成除蟲菊精，共50種待測物，包括歐殺松(acephate)、谷速松(azinphos-methyl)、陶斯松(chlorpyrifos)、內吸磷(demeton)、滅賜松(demeton-S-methyl)、大利松(diazinon)、二氯松(dichlorvos)、大滅松(dimethoate)、二硫松(disulfoton)、普扶松(ethoprophos)、芬殺松(fenthion)、大福松(fonofos)、加福松(isoxathion)、馬拉松(malathion)、達馬拉(methamidophos)、滅大松(methidathion)、美文松(mevinphos)、亞素靈(monocrotophos)、巴拉松(parathion)、賽達松(phenthoate)、福瑞松(phorate)、易滅松(phosmet) 、佈飛松(profenofos)、喹硫磷(quinalphos)、亞培松(temephos)、托福松(terbufos)、三氯松(trichlorfon)、得滅克(aldicarb)、得滅克亞碸(aldicarb sulfoxide)、得滅克碸(aldicarb sulfone)、免扶克(benfuracarb)、加保利(carbaryl)、加保扶(carbofuran)、3-羥基加保扶(3-hydroxycarbofuran)、丁基加保扶(carbosulfan)、愛芬克(ethiofencarb)、丁基滅必蝨(fenobucarb)、滅必蝨(isoprocarb)、滅賜克(methiocarb)、納乃得(methomyl)、歐殺滅(oxamyl)、比加普(pirimicarb)、安丹(propoxur)、賽扶寧(cyfluthrin)、亞滅寧(cypermethrin)、第滅寧(deltamethrin)、芬普寧(fenpropathrin)、芬化利(fenvalerate)、百滅寧(permethrin)、福化利(tau-fluvalinate)，完成前處理技術開發以及液相層析/質譜/質譜儀分析方法建立。 500 mL水樣以Atlantic HLB(47 mm)固相圓盤萃取，以甲醇及二氯甲烷沖提，沖提液經濃縮至5 mL後，以極致液相層析儀(ultra-performance liquid chromatography, UPLC)搭配串聯式質譜儀以電灑游離（ESI）分析。液相層析管柱為Phenomenex Kinetex PFP(50 × 2.1 mm, 2.6 μm)。採樣地點為雲嘉南主要河川北港溪、八掌溪、及鹽水溪之重要支流以及其本流，以及一座水庫、一座淨水廠與一座生活污水處理廠，加上計劃委託單位另外交付之7個水體樣本，共計31個樣本。水體檢測結果顯示，北港溪流域檢出率較高，尤其是機磷類農藥，最高濃度為acephate(9.5 μg/L)。污水廠、淨水廠、水庫與計劃委託單位水樣之待測物濃度皆低於定量極限(LOQ, 500 ng/L)，且大多為未檢出。檢測結果與農藥原體之使用量有正向相關，使用量越高之農藥，待測物於水體中檢出率相對較高。

中文關鍵字

農藥;液相層析串聯式質譜儀;飲用水;表面水;固相萃取

類型	檔名	檔案大小	說明	最新版公開日期
期末報告	EPA-100-E3S4-02-01.pdf	6MB		2012/2/1 上午 12:00:00

摘要	Pesticides have been used widely on agricultural plants, inhibition of weeds, control of pests, etc. Pesticides and their metabolites may enter the water environment through runoff or air deposition, and their impact on human health and aquatic creatures deserve our attentions. Based on the statistical data from the Council of Agriculture, Taiwan, the most used active ingredients among pesticides are insecticides, bactericides, and herbicides. This study developed and validated a method to analyze 50 insecticides using solid-phase extraction and ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS). There are 27 organophosphates, 16 carbamates, and seven pyrethroids, which including acephate, azinphos-methyl, chlorpyrifos, demeton, demeton-S-methyl, diazinon, dichlorvos, dimethoate, disulfoton, ethoprophos, fenthion, fonofos, isoxathion, malathion, methamidophos, methidathion, mevinphos, monocrotophos, parathion, phenthoate, phorate, phosmet, profenofos, quinalphos, temephos, terbufos, trichlorfon, aldicarb, aldicarb sulfoxide, aldicarb sulfone, benfuracarb, carbaryl, carbofuran, 3-hydroxycarbofuran, carbosulfan, ethiofencarb, fenobucarb, isoprocarb, methiocarb, methomyl, oxamyl, pirimicarb, propoxur, cyfluthrin, cypermethrin, deltamethrin, fenpropathrin, fenvalerate, permethrin, and tau-fluvalinate. 500-mL of water was extracted with Atlantic HLB disk (47 mm) and was eluted with methanol and dichloromethane. The combined eluent was concentrated to 5 mL and was analyzed with the UPLC-MS/MS at positive electrospray ionization. A Phenomenex Kinetex PFP (50 × 2.1 mm, 2.6 μm) core-shell column was used for the chromatography. Samples were collected from three rivers of southern Taiwan (Beigang River, Bajhang River, and Yanshuei River), a reservoir for a drinking water plants, drinking water, and a sewage treatment plant, plus seven drinking water samples from the National Institute of Environmental Analysis (NIEA), in the total of 31 samples. The positive rate was the highest in Beigang River among the three rivers, especially on organophosphates (the highest was acephate at 9.5 μg/L). The detected concentrations were associated with the used amount of pesticide active ingredients. Concentrations of analytes in the samples from the sewage treatment plant, the drinking water treatment plant, the reservoir, and the samples from the NIEA were either lower than the limit of quantitation (500 ng/L) or not detectable.
英文關鍵字	Pesticides;LC-MS/MS;drinking water;Surface water;Solid-phase extraction

摘要

Pesticides have been used widely on agricultural plants, inhibition of weeds, control of pests, etc. Pesticides and their metabolites may enter the water environment through runoff or air deposition, and their impact on human health and aquatic creatures deserve our attentions. Based on the statistical data from the Council of Agriculture, Taiwan, the most used active ingredients among pesticides are insecticides, bactericides, and herbicides. This study developed and validated a method to analyze 50 insecticides using solid-phase extraction and ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS). There are 27 organophosphates, 16 carbamates, and seven pyrethroids, which including acephate, azinphos-methyl, chlorpyrifos, demeton, demeton-S-methyl, diazinon, dichlorvos, dimethoate, disulfoton, ethoprophos, fenthion, fonofos, isoxathion, malathion, methamidophos, methidathion, mevinphos, monocrotophos, parathion, phenthoate, phorate, phosmet, profenofos, quinalphos, temephos, terbufos, trichlorfon, aldicarb, aldicarb sulfoxide, aldicarb sulfone, benfuracarb, carbaryl, carbofuran, 3-hydroxycarbofuran, carbosulfan, ethiofencarb, fenobucarb, isoprocarb, methiocarb, methomyl, oxamyl, pirimicarb, propoxur, cyfluthrin, cypermethrin, deltamethrin, fenpropathrin, fenvalerate, permethrin, and tau-fluvalinate. 500-mL of water was extracted with Atlantic HLB disk (47 mm) and was eluted with methanol and dichloromethane. The combined eluent was concentrated to 5 mL and was analyzed with the UPLC-MS/MS at positive electrospray ionization. A Phenomenex Kinetex PFP (50 × 2.1 mm, 2.6 μm) core-shell column was used for the chromatography. Samples were collected from three rivers of southern Taiwan (Beigang River, Bajhang River, and Yanshuei River), a reservoir for a drinking water plants, drinking water, and a sewage treatment plant, plus seven drinking water samples from the National Institute of Environmental Analysis (NIEA), in the total of 31 samples. The positive rate was the highest in Beigang River among the three rivers, especially on organophosphates (the highest was acephate at 9.5 μg/L). The detected concentrations were associated with the used amount of pesticide active ingredients. Concentrations of analytes in the samples from the sewage treatment plant, the drinking water treatment plant, the reservoir, and the samples from the NIEA were either lower than the limit of quantitation (500 ng/L) or not detectable.

英文關鍵字

Pesticides;LC-MS/MS;drinking water;Surface water;Solid-phase extraction