This study reviews the latest literatures to bridge the domestic knowledge gaps on measurement and characterization of nanomaterials, and exposure and risk assessments of nanomaterials. Workshops on these topics are also held and the suggestions and comments from the participating experts are collected. This study also gathers the developing tendency and important information of environmental, health, and safety (EHS) issues on nanotechnology from International Organization for Standardization (ISO) and Organization for Economic Co-operation and Development (OECD). The knowledge contents is also provided for another EPA project “Consolidation and Maintenance of Environmental Nanotechnology Knowledge Platform” and exchanged with the experts. Through the discussion on the forum and platform, Nanotechnology EHS issues can be exchanged and spread to the general public.