Responding to implementation of the regulations of existing chemical substances standard registration, this project has collected the physiochemical, toxicological and eco-toxicological data of the designated existing chemical substances subject to standard registration. The international open access databases which apply to data collection were mainly from the guidance on existing chemical substances standard registration. This analysis report covered citation indexes, copyright information, and others according to substances properties. Also, some collection results were data lacking, therefore, the strategies of data gap filling for five typical substances, including an organic compound, an inorganic compound, an acid substance, a base substance, and a metal compound, have been conducted. In addition, this project not only shows the differences between National Standards of the Republic of China (CNS) 15030 and Globally Harmonized System of Classification and Labeling of Chemicals eight revised edition (GHS Rev.8) but also provides the advice to the preparation and application of the information of existing chemical substances standard registration in accordance with the physical, health, and environment hazards. At the same time, the hazard information transmission, based on GHS hazard identification, and the hazard classification methods of mixtures have been proposed. This project aims to strengthen the management of existing chemicals in accordance with the registration system of chemical substances. Three testing proposal examples of toxicology or eco-toxicology as the references for registrants have been finished as well.