The analyses were distinguished by chemical analysis and bioassay. Water and sediment samples were taken along Kaoping Rive and estuary. Fractions of household commodities were also analyzed for Nonylphenol (NP) or tribulytin (TBT). The sample sizes totaled 81 for NP test, 44 for river water, 20 for groundwater, 10 for sediment, 4 for household commodities, and 3 for emulsion respectively. The sample sizes totaled 39 for TBT test, 20 for seawater, 6 for sediment, 9 for fish muscular, and 4 for fishnet, respectively.
NP concentration for water samples, sediment, and emulsion agent were 0.19~183.4 mg/l, 134.7-354.4 ng/g, and 1.9-13.4 mg/g, respectively. The TBT analytic results showed that 25% of the samples contained TBT. Samples for sediment contained 83.3% of TBT. The concentration of TBT for water samples and sediment were ＜0.0026~83.2 ng/l，and ＜1.24~72.39 ng/g. The TBT concentration in Fish Port of Shann-Wei was much greater than other sampling locations. Samples of fish muscular contained 100% of TBT residuary that reached a concentration between 26.4 and 194.2 ng/g.
Bioassay MVLN investigated estrogenic activity substance of the samples. MCF-AR1 examined androgen-like substance. 84 samples taken from Kaoping Rive were analyzed by both bioassay methods. The results form estrogenic activity analysis showed that 4.8% of the samples belonged to full agonist and 7.1% of the samples belonged to partial agonist. As for androgen activity analysis, it showed that 4.8% of samples belonged to partial agonist and none of samples belonged to full agonist.
Both NP and TBT were begun on risk assessment. The measured concentration was divided by Predicted No-Effect Concentration (PNEC) to obtain ecotoxicity hazard quotient. Human risk characterization was evaluated for Margin of Safety (MOS) that accepted daily intake/measured daily intake. The results of risk characterization showed that the levels of NP in both water and sediment might cause potential risks to aquatic and benthonic organisms. However, more samples were needed to verify potential TBT risk.